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Principle Of Urine Analyzer
- Sep 04, 2018 -

Such instruments are generally controlled by a microcomputer, and the color change on the test strip is measured by a spherical area meter to receive a two-wavelength reflected light for semi-quantitative determination. There are several reagent pads containing various reagents on the reagent strip, each of which reacts independently with the corresponding components in the urine, and displays different colors. The color depth is proportional to a certain component in the urine, and there is another in the reagent strip. The compensation pad", as the color of the urine background, compensates for errors caused by colored urine and instrument changes.

The reagent strip with the adsorbed urine is placed in the colorimetric cell of the instrument, and various reagent pads on the reagent strip that have generated a chemical reaction are irradiated by the light source, and the reflected light is received by the sphere area meter, and the photocell of the sphere area meter is reflected. The two-wavelength light (measured by the measurement light of the filter and a reference beam of light) is irradiated, and the selection of each wavelength is determined by the detection item.

The instrument automatically calculates the reflectance according to the following formula, and then compares it with the standard curve to automatically find the corresponding results of various components. The content of a certain component in the urine is high, and the reflected light of the corresponding reagent pad is dark, otherwise it is strong.

Reflectance fraction: R (%) = Tm. Cs / TsCm × 100%

R(%) in the formula is the reflectance; Tm is the reflection intensity of the reagent pad to the measurement wavelength; Ts is the reflection intensity of the reagent pad to the reference wavelength; Cm is the reflection intensity of the alignment pad to measure the length of the ; Cs is the calibration pair The intensity of the reflection of the reference wavelength.